Basic Information

Dataset GSE171199
Status 2022-04-26
Species Mus musculus
Source NCBI GEO
Subcellular Localization Neurite|Soma
PMID 35438793
Title m6A and YTHDF proteins control the subcellular localization of select neuronal mRNAs [RNA-seq and DART-seq]
Citation Flamand MN, Meyer KD. m6A and YTHDF proteins contribute to the localization of select neuronal mRNAs. Nucleic Acids Res 2022 May 6;50(8):4464-4483.
Experimental Design RNA abundance measurement and m6A sites profiling in the soma and neurite compartments of wildtype and Mettl3-/- hippocampal neurons. Primary neurons were grown on microporous membrane and treated with KCl for 2 hours. Total RNA was extracted from the top (soma) and bottom (neurite) of the membrane and used for RNA-seq or in vitro DART-seq library preparation in duplicates.

Sample Information

BioProject Sample Name Localization Treatment Genotype Source Label
PRJNA718617 GSM5220658 Soma untreated Mettl3-/- Hippocampal Neurons Mettl3_KO_Soma_Ctrl_rep1
PRJNA718617 GSM5220659 Neurite untreated Mettl3-/- Hippocampal Neurons Mettl3_KO_Neurite_Ctrl_rep1
PRJNA718617 GSM5220660 Soma Treated with KCl for 2 h. Mettl3-/- Hippocampal Neurons Mettl3_KO_Soma_KCl_rep1
PRJNA718617 GSM5220661 Neurite Treated with KCl for 2 h. Mettl3-/- Hippocampal Neurons Mettl3_KO_Neurite_KCl_rep1
PRJNA718617 GSM5220662 Soma untreated WT Hippocampal Neurons WT_Soma_Ctrl_rep1
PRJNA718617 GSM5220663 Neurite untreated WT Hippocampal Neurons WT_Neurite_Ctrl_rep1
PRJNA718617 GSM5220664 Soma untreated WT Hippocampal Neurons WT_Soma_Ctrl_rep2
PRJNA718617 GSM5220665 Neurite untreated WT Hippocampal Neurons WT_Neurite_Ctrl_rep2
PRJNA718617 GSM5220666 Soma Treated with KCl for 2 h. WT Hippocampal Neurons WT_Soma_KCl_rep1
PRJNA718617 GSM5220667 Neurite Treated with KCl for 2 h. WT Hippocampal Neurons WT_Neurite_KCl_rep1
PRJNA718617 GSM5220668 Soma Treated with KCl for 2 h. WT Hippocampal Neurons WT_Soma_KCl_rep2
PRJNA718617 GSM5220669 Neurite Treated with KCl for 2 h. WT Hippocampal Neurons WT_Neurite_KCl_rep2
PRJNA718617 GSM5220670 Soma untreated Mettl3-/- Hippocampal Neurons Mettl3_KO_Soma_Ctrl_rep2
PRJNA718617 GSM5220671 Neurite untreated Mettl3-/- Hippocampal Neurons Mettl3_KO_Neurite_Ctrl_rep2
PRJNA718617 GSM5220672 Soma Treated with KCl for 2 h. Mettl3-/- Hippocampal Neurons Mettl3_KO_Soma_KCl_rep2
PRJNA718617 GSM5220673 Neurite Treated with KCl for 2 h. Mettl3-/- Hippocampal Neurons Mettl3_KO_Neurite_KCl_rep2

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