Basic Information
| Dataset | GSE154494 |
|---|---|
| Status | 2021-01-31 |
| Species | Homo sapiens |
| Source | NCBI GEO |
| Subcellular Localization | Cytoplasmic (NES)|Nuclear (NLS) |
| PMID | 34326467 |
| Title | Next Generation Sequencing of MDA-MB-231 cells with variable subcellular localization of Aurora-A Kinase (Wild-type (WT), no localization signal sequence, Cytoplasmic (NES) or Nuclear (NLS)) . |
| Citation | Whately KM, Voronkova MA, Maskey A, Gandhi J et al. Nuclear Aurora-A kinase-induced hypoxia signaling drives early dissemination and metastasis in breast cancer: implications for detection of metastatic tumors. Oncogene 2021 Sep;40(37):5651-5664. |
| Experimental Design | The mRNA profiles of MDA-MB-231 cells with variable subcellular localization of Aurora-A Kinase in Cytoplasm or Nucleus or both were generated by deep sequencing, in triplicate, using Illumina HiSeq and correlated with metstatic potenital of these cell in vivo xenograft studes. |
Sample Information
| BioProject | Sample Name | Localization | Genotype | Source | Label |
|---|---|---|---|---|---|
| PRJNA646478 | GSM4672196 | no localization signal sequence | Empty SgCon | MDA-MB-231 cells | E) Empty SgCon |
| PRJNA646478 | GSM4672197 | no localization signal sequence | Empty SgCon | MDA-MB-231 cells | I) Empty SgCon |
| PRJNA646478 | GSM4672198 | no localization signal sequence | Empty SgCon | MDA-MB-231 cells | J) Empty SgCon |
| PRJNA646478 | GSM4672199 | Cytoplasmic (NES) | Empty SgCon | MDA-MB-231 cells | C) NES-Empty SgCon |
| PRJNA646478 | GSM4672200 | Cytoplasmic (NES) | Empty SgCon | MDA-MB-231 cells | K) NES-Empty SgCon |
| PRJNA646478 | GSM4672201 | Cytoplasmic (NES) | Empty SgCon | MDA-MB-231 cells | L) NES-Empty SgCon |
| PRJNA646478 | GSM4672202 | Nuclear (NLS) | Empty SgCon | MDA-MB-231 cells | G) NLS-Empty SgCon |
| PRJNA646478 | GSM4672203 | Nuclear (NLS) | Empty SgCon | MDA-MB-231 cells | M) NLS-Empty SgCon |
| PRJNA646478 | GSM4672204 | Nuclear (NLS) | Empty SgCon | MDA-MB-231 cells | N) NLS-Empty SgCon |
| PRJNA646478 | GSM4672205 | Wild-type (WT) | AURKA c1 | MDA-MB-231 cells | D) WT-AURKA c1 |
| PRJNA646478 | GSM4672206 | Wild-type (WT) | AURKA c1 | MDA-MB-231 cells | O) WT-AURKA c1 |
| PRJNA646478 | GSM4672207 | Wild-type (WT) | AURKA c1 | MDA-MB-231 cells | P) WT-AURKA c1 |
| PRJNA646478 | GSM4672208 | Wild-type (WT) | AURKA c2 | MDA-MB-231 cells | Q) WT-AURKA c2 |
| PRJNA646478 | GSM4672209 | Wild-type (WT) | AURKA c2 | MDA-MB-231 cells | R) WT-AURKA c2 |
| PRJNA646478 | GSM4672210 | Cytoplasmic (NES) | AURKA c1 | MDA-MB-231 cells | F) NES-AURKA c1 |
| PRJNA646478 | GSM4672211 | Cytoplasmic (NES) | AURKA c1 | MDA-MB-231 cells | S) NES-AURKA c1 |
| PRJNA646478 | GSM4672212 | Cytoplasmic (NES) | AURKA c1 | MDA-MB-231 cells | T) NES-AURKA c1 |
| PRJNA646478 | GSM4672213 | Cytoplasmic (NES) | AURKA c2 | MDA-MB-231 cells | A) NES-AURKA c2 |
| PRJNA646478 | GSM4672214 | Cytoplasmic (NES) | AURKA c2 | MDA-MB-231 cells | U) NES-AURKA c2 |
| PRJNA646478 | GSM4672215 | Cytoplasmic (NES) | AURKA c2 | MDA-MB-231 cells | V) NES-AURKA c2 |
| PRJNA646478 | GSM4672216 | Nuclear (NLS) | AURKA c2 | MDA-MB-231 cells | H) NLS-AURKA c2 |
| PRJNA646478 | GSM4672217 | Nuclear (NLS) | AURKA c2 | MDA-MB-231 cells | W) NLS-AURKA c2 |
| PRJNA646478 | GSM4672218 | Nuclear (NLS) | AURKA c2 | MDA-MB-231 cells | X) NLS-AURKA c2 |
| PRJNA646478 | GSM4672219 | Nuclear (NLS) | AURKA c3 | MDA-MB-231 cells | B) NLS-AURKA c3 |
| PRJNA646478 | GSM4672220 | Nuclear (NLS) | AURKA c3 | MDA-MB-231 cells | Y) NLS-AURKA c3 |
| PRJNA646478 | GSM4672221 | Nuclear (NLS) | AURKA c3 | MDA-MB-231 cells | Z) NLS-AURKA c3 |
Subcellular Localization Number
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Condition:
Localization Differential Analysis
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Enrichment:
Specific Subcellular Localization
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Dynamic RNA Localization
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Method:
Specific Enrichment Analysis
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Enrichment:
Specific RNA Ratio
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TPM:
