Basic Information
| Dataset | GSE106862 |
|---|---|
| Status | 2018-05-07 |
| Species | Rattus norvegicus |
| Source | NCBI GEO |
| Subcellular Localization | soma|protrusion |
| PMID | 29732611 |
| Title | The oligodendrocyte "protrusiosome": Identification of compartmentalized enrichment of transcripts in newly-formed protrusions of oligodendrocyte precursor cells (OPC) by RNAseq |
| Citation | Azevedo MM, Domingues HS, Cordelières FP, Sampaio P et al. Jmy regulates oligodendrocyte differentiation via modulation of actin cytoskeleton dynamics. Glia 2018 Sep;66(9):1826-1844. |
| Experimental Design | Total RNA from OPC soma and membrane protrusions was isolated using the Quick-RNA MicroPrep kit (Zymo Research) according with manufacturer’s instructions. The OPC soma fraction was isolated by scrapping the upper side of the insert with a cotton swab. Total RNA was further isolated from the cotton swab by dipping it in ZR RNA lysis buffer. RNA from membrane protrusions was collected from the bottom side of the insert, which was pushed down into a well containing lysis buffer. RNA from different experiments was pooled and concentrated using the RNA Clean & Concentrator-5 kit (Zymo Research). Total RNA quantity and integrity were assessed using Quant-IT RiboGreen RNA Assay Kit (Invitrogen, Carlsbad, CA, USA) and Agilent Tapestation 2200 R6K. mRNA enrichment was achieved by processing 5 μg of total RNA using the Magnetic mRNA Isolation Kit from NEB (S1550S) with minor modifications. |
Sample Information
| BioProject | Sample Name | Localization | Develop Stage | Source | Label |
|---|---|---|---|---|---|
| PRJNA418248 | GSM2856133 | soma | P0-P2 | oligodendrocyte precursor cells (OPC) | OPC soma_1 |
| PRJNA418248 | GSM2856134 | soma | P0-P2 | oligodendrocyte precursor cells (OPC) | OPC soma_2 |
| PRJNA418248 | GSM2856135 | protrusion | P0-P2 | oligodendrocyte precursor cells (OPC) | OPC protrusion_1 |
| PRJNA418248 | GSM2856136 | protrusion | P0-P2 | oligodendrocyte precursor cells (OPC) | OPC protrusion_2 |
Subcellular Localization Number
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Localization Differential Analysis
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Specific Subcellular Localization
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Dynamic RNA Localization
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Specific Enrichment Analysis
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Specific RNA Ratio
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